In-vitro efficacy evaluation of ethanolic extract of Cinnamomum zeylanicum (cinnamon) as potential antimicrobial agent against clinical isolates

Author(s) : Fizza Riaz, Rizwan Asif, Naureen Naeem, Madiha Mustafa, Minhas Alim, Zaigham Wahhab & Ayesha Riaz

Abstract:

DOI: https://doi.org/10.52587/njhms.v6i1.77

Objective:
Diabetes mellitus (DM) is a metabolic disorder with increase prevalence around the globe and linked with multiple complications. Diabetic foot ulcer is most lethal complication of diabetes mellitus (DM). The aim of this study is to determine the microbiology profile of diabetic foot ulcers and the antimicrobial susceptibility of Cinnamomum zeylanicum (Darchini) against isolated bacteria.
Material and Methods:
This study was conducted on 100 patients with diabetic foot ulcer from different diabetic centers of Lahore, Punjab. Samples were obtained from wound using sterilized techniques. Different microbiological techniques like colony morphology, specific media, gram staining and biochemical parameters were used to isolate and identified the bacteria. Disc diffusion and well-diffusion methods were performed for antimicrobial susceptibility of standards antibiotics and cinnamon extract.
Results:
Various bacteria like S. aureus (78), P. aeruginosa (50), E. coli (44), K. pneumonia (28) P. mirabilis (14), S. epidermidis (17) were identified on the base of colony morphology, gram staining and biochemical testing. . Antibacterial analysis revealed sensitivity of the C. zeylanicum extract against S. aureus (12mm), P. aeruginosa (50), E. coli (44), K. pneumonia (28) P. mirabilis (14), S. epidermidis (17). Selected extracts against isolates showed significant (p<0.05) antimicrobial activity comparable to synthetic antibiotics.
Conclusion:
The lemon extract showed moderate antibacterial activity against S. epidermidis (8mm), P. aeruginosa (10mm) Bacillus (10mm), and P. mirabilis (11mm). Similarly, pomegranate also showed moderate sensitivity against all isolated bacteria. Jamun leaf extract showed antibacterial activity against S. epidermidis (12mm), K. pneumonia (10mm), E. coli (12mm), S. aureus, (11mm) and P. mirabilis (10mm). Hence plants may be used as an alternative to synthetic antibiotics with modifications to achieve better results.

Keywords: Diabetic foot, Bacterial profile, C. zeylanicum, Antibacterial Potential