In vitro thrombolytic and cytoprotective potential of Moringa oleifera leaves extract
Author(s) : Muhammad Afzal, Muhammad Shahid and Fatima Yousaf
Hemostasis is the natural process causing the blood to stop flowing out from damaged blood vessel following an injury. This involves the coagulation process activating the coagulation mechanism to form fibrin clot. Cytotoxicity is the ability of an agent to destroy the living cells. The current work was aimed to study the thrombolytic and cytoprotective potential of Moringa oleifera leaves extract through in vitro assays.
Material and Methods:
M. oleifera leaves extract was tested for thrombolytic activity against human blood clots through clot lysis assay using streptokinase as standard thrombolytic agent. For evaluation of cytoprotective potential of the aqueous leaves extract, hemolytic assay was performed using washed human erythrocytes. Distilled water and Triton X-100 was used as negative and positive controls, respectively.
Current study results showed significant (p<0.05) clot lysis activity against blood clots but the percent clot lysis by M. oleifera extract (42.74±1.8%) was lower than that of standard streptokinase (79.4±2.6%). No significant (p>0.05) hemolysis was observed in M. oleifera leaves extract (6.58±1.02%) treated red cells and distilled water (2.84±0.78%) while remarkably significant (p<0.05) hemolysis of washed red cells incubated with triton X-100 (86.04 ± 4.87 %) was observed.
The study indicated that M. oleifera leaves have therapeutic potential against blood clots with less cytotoxic effect on human erythrocytes. Hence, this plant might be used as cardioprotective and membrane stabilizing agents in pharmaceutical preparations after proper screening and analysis of bioactives present in aqueous extract of this plant.
Keywords: Thrombolytic, streptokinase, cardioprotective, cytotoxicity