Detection of Serum Associated Proteins as Diagnostic Biomarkers in Patients suffering from Lung Cancer

Author(s) : Samina, Junaid Ahmed, Shabir Ahmad Khan, Roha Talat, Naseeb Ullah, Mohsin Ali, Iqra Ayub, Farah Sabeen Bugti & Mohammad Zahid Mustafa

Abstract:

Objective:
Lung cancer (LC) is one of the leading causes of cancer related deaths worldwide. Among other reasons, one cause of LC deaths is the lack of early diagnosis. Serum biomarkers might be helpful in early detection of this cancer but there is no up to date specific biomarker reported to diagnose LC. Aim of the present study was to predict the tumor associated proteins as potential biomarkers for the early detection of Lung Cancer (LC).
Material and Methods:
50 serum samples from LC patients were tested and SDS-PAGE profiling was performed. The visualization of detected protein bands the gels were stained by Coomassie blue staining method.
Results:
Two bands were differentially expressed by LC patients comparing control sera. A 41 kDa band was stained in 21% testing sera while 110 kDa band was stained with 17% testing sera of patients.
Conclusion:
These protein bands might serve as candidate potential biomarkers for early LC diagnosis after mass spectrometry confirmation with large scale testing sera.
Keywords: Sera, Diagnostic biomarker, Lung cancer